CellaVision Global Test 2026:3 – Primary Myelofibrosis (PMF)

 

Case Description

64-year-old man with a 4-week history of increasing fatigue, weakness, night sweats and unintentional weight loss. He reports intermittent low-grade fever. No previous history of hematologic disease. 

On examination he appears pale and fatigued, with a swollen abdomen.

Laboratory testing showed a slight leukocytosis (WBC 12.6 × 10⁹/L), thrombocytopenia (PLT 58 × 10⁹/L) and slightly anemic (HGB 100 g/L). The automated differential showed an increased basophil count, and the analyzer flags indicated “Blasts?”, “IG present?”, and “Atypical lymph?”.

Laboratory Findings

Test 

Result 

Reference (male) 

Units 

WBC 

12.64

4.0 - 10.0

 x109/L

RBC 

3.52

 4.5 – 5.9

x1012/L

HGB

100

 134 – 170 g/dL

HCT

0.30

 0.4-0.5

L/L
PLT 58 150  –  400 x109/L

Cell Counter Differential

Test 

Result (x109/L)

Result (%)

Neutrophils

8.72

 69

Lymphocytes

2.66

15.5

Monocytes

0.66

 5.2

Eosinophils

0.19

1.5
Basophils 0.41 3.3

WBC flags: Blasts, Atyp lymph, IG Present, NRBC present

Analysis Details 

Stain: May–Grünwald–Giemsa

Analyzer: CellaVision DC-1, CDMS 7.2, LED Microscope mode

Sample of cells classified in the Global Test 2026:3  

Sample of cells classified in the Global test 2026:2

RBC Overview:

RBC Overview Global Test 2026:3

Smear Interpretation

Peripheral blood smear examination reveals increased myelopoiesis’s with immature granulocytes (12%) and increased basophil count present. 

Additional findings include Nucleated RBCs in different maturation steps as well as schistocytes, teardrop cells, and elliptocytes. 

Diagnosis

Primary Myelofibrosis (PMF) is a rare blood cancer belonging to a group of disorders called Myeloproliferative Neoplasms (MPNs). In its overt fibrotic stage, PMF is highly characterized by a leukoerythroblastic blood picture, featuring an increase of immature granulocytes and nucleated red blood cells in the peripheral blood smear, as well as a high number of teardrop cells (dacrocytes). 

Global Test 2026:3 Results Webinar

  • Case presentation
  • DC-1 Analysis - the WBC differential and RBC Characterization
  • Differences in classification between examiner and participants
  • Discussion about Primary Myelofibrosis  – From symptoms to laboratory diagnosis

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