Kit RAL StainBox BBM
Ref. 360400
Description
Kit RAL StainBox BBM
The BBM panoptic staining allows to perform blood cell and bone marrow counting, it is realized by using successively five reagents: R1, R2, R3, R4 and R5. R1, formulated with ethylic alcohol, is a mixture of neutral stains. It allows the smear fixation and prepares the staining, especially of hydrosoluble elements such as basophilic granules.
Those stains are inactive in alcoholic medium, and only react selectively when released in R2 and R3 solutions. This releasing generates the precipitation of neutral stains, leading to the staining of erythrocytes, cytoplasm of neutrophilic granulocytes as well as eosinophilic granules. R4 is a blue aqueous solution which stains the cytoplasm of monocytes and lymphocytes. R4 also eases the metachromasia process as it colors azurophilic granules red. Eventually, R5 removes excess of stain and participates to the differentiation of cellular elements thanks to the action of specially selected rinsing agents.
The successive action of R1, R2, R3, R4 and R5 brings the violet color (typical Romanowsky-Giemsa effect), particularly visible in chromatin, platelets, and neutrophilic granules.
Components
| Name | Reference | Packing |
|---|---|---|
| R1 | 313595-0250 | 230 mL |
| R2 | 3135752A0250 | 230 mL |
| R3 | 3135753A0250 | 230 mL |
| R4 | 313565-0250 | 230 mL |
| R5 | 313605-0250 | 230 mL |
Kit RAL StainBox BBM
The BBM panoptic staining allows to perform blood cell and bone marrow counting, it is realized by using successively five reagents: R1, R2, R3, R4 and R5. R1, formulated with ethylic alcohol, is a mixture of neutral stains. It allows the smear fixation and prepares the staining, especially of hydrosoluble elements such as basophilic granules.
Those stains are inactive in alcoholic medium, and only react selectively when released in R2 and R3 solutions. This releasing generates the precipitation of neutral stains, leading to the staining of erythrocytes, cytoplasm of neutrophilic granulocytes as well as eosinophilic granules. R4 is a blue aqueous solution which stains the cytoplasm of monocytes and lymphocytes. R4 also eases the metachromasia process as it colors azurophilic granules red. Eventually, R5 removes excess of stain and participates to the differentiation of cellular elements thanks to the action of specially selected rinsing agents.
The successive action of R1, R2, R3, R4 and R5 brings the violet color (typical Romanowsky-Giemsa effect), particularly visible in chromatin, platelets, and neutrophilic granules.
| Name | Reference | Packing |
|---|---|---|
| R1 | 313595-0250 | 230 mL |
| R2 | 3135752A0250 | 230 mL |
| R3 | 3135753A0250 | 230 mL |
| R4 | 313565-0250 | 230 mL |
| R5 | 313605-0250 | 230 mL |
Reagents Technical Documentation
On https://my.ral-diagnostics.fr you get access to all our reagents documentation and media.
