Kit RAL StainBox MCDh
Ref. 360300
Description
Fixation and differential staining of cellular structures
The MCDh panoptic staining allows to perform blood cell counting, realized by using successively four reagents: MCDh 1, MCDh 2, MCDh 3 and MCDh 4.
MCDh 1, formulated with ethylic alcohol, is a mixture of neutral stains. It allows a smear fixation and prepares the staining, especially the one of hydrosoluble elements such as basophilic granules. Those stains are inactive in alcoholic medium, and only react selectively when released in MCDh 2 solution. This releasing generates the precipitation of neutral stains, leading to erythrocytes, cytoplasm of neutrophilic granulocytes as well as eosinophilic granules staining. MCDh 3 is an aqueous solution which stains cytoplasm of monocytes and lymphocytes. MCDh 3 also eases the metachromasia process as it colors azurophilic granules red. Eventually, MCDh 4 removes excess of stain and participates to differentiation of cellular elements thanks to action of specially selected rinsing agents.
The successive action of MCDh 1, MCDh 2, MCDh 3 and MCDh 4 brings the violet color (typical Romanowsky-Giemsa effect), particularly visible in chromatin, platelets and neutrophilic granules.
Information Request
Want to learn more about our product concept, request a demonstration, or just get in touch with CellaVision or your local CellaVision distributor? Contact us at commercial@cellavision.com.
Components
| Name | Reference | Packing |
|---|---|---|
| MCDh 1 | 313590-0250 | 1 x 230 mL |
| MCDh 2 | 3135702A250 / 3135703A250 | 1 x 230 mL / 1 x 230 mL |
| MCDh 3 | 313560-0250 | 1 x 230 mL |
| MCDh 4 | 313600-0250 | 4 x 230 mL |
Fixation and differential staining of cellular structures
The MCDh panoptic staining allows to perform blood cell counting, realized by using successively four reagents: MCDh 1, MCDh 2, MCDh 3 and MCDh 4.
MCDh 1, formulated with ethylic alcohol, is a mixture of neutral stains. It allows a smear fixation and prepares the staining, especially the one of hydrosoluble elements such as basophilic granules. Those stains are inactive in alcoholic medium, and only react selectively when released in MCDh 2 solution. This releasing generates the precipitation of neutral stains, leading to erythrocytes, cytoplasm of neutrophilic granulocytes as well as eosinophilic granules staining. MCDh 3 is an aqueous solution which stains cytoplasm of monocytes and lymphocytes. MCDh 3 also eases the metachromasia process as it colors azurophilic granules red. Eventually, MCDh 4 removes excess of stain and participates to differentiation of cellular elements thanks to action of specially selected rinsing agents.
The successive action of MCDh 1, MCDh 2, MCDh 3 and MCDh 4 brings the violet color (typical Romanowsky-Giemsa effect), particularly visible in chromatin, platelets and neutrophilic granules.
Information Request
Want to learn more about our product concept, request a demonstration, or just get in touch with CellaVision or your local CellaVision distributor? Contact us at commercial@cellavision.com.
| Name | Reference | Packing |
|---|---|---|
| MCDh 1 | 313590-0250 | 1 x 230 mL |
| MCDh 2 | 3135702A250 / 3135703A250 | 1 x 230 mL / 1 x 230 mL |
| MCDh 3 | 313560-0250 | 1 x 230 mL |
| MCDh 4 | 313600-0250 | 4 x 230 mL |
Reagents Technical Documentation
On https://my.ral-diagnostics.fr you get access to all our reagents documentation and media.
