Lymphopoiesis

Lymphoblast

  • 10-20 microns
  • Round to oval nucleus with fine, evenely stained chromatin structure, 1-2 nucleoles
  • Blue cytoplasm with clear zone closest to the nucleus
  • No granules
 

Prolymphocyte

  • 10-20 microns
  • Round nucleus, somewhat looser structure than the lymphoblast. One (1) distinct nucleol
  • Blue cytoplasmc
  • No granules
 

Small Lymphocyte

  • 7-8 microns
  • Round to oval nucleus, condensed chromatin structure. No nucleoles.
  • Narrow basophilic (light or dark blue) cytoplasm band
  • Few bright violet granules
 

Large Lymphocyte

  • 9-15 microns
  • Oval/bulging nucleus, somewhat looser chromatin structure than small lymphocyte. No nucleoles
  • Plentiful colorless to light blue cytoplasm
  • Few bright violet granules
 

Plasma Cell

  • 7-15 microns
  • Round nucleus, eccentrically placed. Compact (radial) chromatin structure
  • Dark blue cytoplasm with perinuclear zone (sometimes vacuoles)
 

Megakarypoiesis

Megakaryoblast

    • The cell that gives rise to the megakaryocyte is the megakaryoblast, which is difficult to identify morphologically
 
 

Megakaryocyte

  • 25-90 microns
  • Round, irregular cell wal
  • Nucleus with 2-16 lobes. The size of the cell varies according to number of lobes.
  • Finely dispersed chromatin mesh in the nucleus. Small nucleoles may be present.
  • Blue to pink cytoplasm
 
 

Thrombocytes

  • 1-15 micron
  • Cytoplasm fragment of the megakaryocyt
  • Light blue to colorless cytoplas
  • Scattered red granules
 
 

Monopoiesis

Promonocyte

  • 15-20 microns
  • Irregularly shaped nucleus without lobes. Fine chromatin structure with nucleoles
  • Gray-blue cytoplasm
  • A few granules can occur
 
 

Monocyte

  • 12-24 microns
  • Kidney-shaped, bulging or segmented nucleus, very loose chromatin structure
  • Powder-fine, violet granules can occur
  • Vacuoles often appear in the cytoplasm
 

Myelopoiesis

Myeloblast

  • 15-20 microns
  • Large round nucleus in the middle of the cell, thin, fine chromatin structure, 2-5 nucleoles
  • Relatively small amount of blue cytoplasm, sometimes with a clear zone
  • No granules
 

Promyelocyt

  • 12-24 microns
  • Oval, possibly slightly bulging nucleus, often with eccentric position. Slightly coarser chromatin structure than myeloblast. 3-4 visible nucleoles
  • Plentiful pale blue cytoplasm
  • Moderate to plentiful unevenly sized violet primary granules
 

Myelocyte

  • 10-18 microns
  • Round to oval nucleus, often eccentrically placed.
  • Coarse and more condensed chromatin structure. No nucleoles
  • Plentiful pink to colorless cytoplasm (more immature, bluish)
  • Very fine moderate to dense granulation (secondary granules)
  • Neutrophilic myelocyte: Violet granules
  • Eosinophilic myelocyte: Bright pink granules
  • Basophilic myelocyte: Blue-violet (black) granules
 

Metamyelocyte

  • 10-16 microns
  • Indented nucleus, coarse clumped chromatin structure. No nucleoles.
  • Pale pink to colorless cytoplasm
  • Many secondary granules
  • Neutrophilic metamyelocyte: Violet granules
  • Eosinophilic metamyelocyte: Pink granules
  • Basophilic metamyelocyte: Blue-violet (black) granules
 

Band Neutrophil Granulocyte

  • 10-15 microns
  • Band shaped nucleus with coarse, clumped chromatin structure. The narrowest part is at least 1/3 of the widest. No nucleoles
  • Pale pink to colorless cytoplasm
  • Plentiful very fine violet granules
 

Segmented Neutrophil Granulocyte

  • 10-15 microns
  • Coarse, clumped chromatin structure. 3-5 lobes. No nucleoles.
  • Pale pink to colorless cytoplasm
  • Abundant secondary violet granules
 

Eosinophil Granulocyte

  • 10-15 microns
  • Coarse, clumped chromatin structure.2-3 lobes. No nucleoles
  • Pale pink to colorless cytoplasm
  • Abundant secondary brick-red granules
 

Basophil Granulocyte

  • 10-15 microns
  • Coarse, clumped chromatin structure. 3-4 lobes. No nucleoles
  • Pale pink to colorless cytoplasm.
  • Secondary blue-violet (black) uneven distributed granules
 

Erythropoiesis

Proerythroblast

  • 14-24 microns
  • Large round centrally placed nucleos with fine chromatin structure. 2-5 nucleoles.
  • Narrow dark blue cytoplasm edge. Uneven structure with well-defined perinuclear clear zone
  • No granules
 
 

Basophilic Erythroblast

  • 10-17 microns
  • Round rather small nucleos. Slightly condensed chromatin structure. No nucleoles
  • Wider dark blue cytoplasm edge
  • No granules
 
 

Polychromatic Erythroblast

  • 10-15 microns
  • Round, even smaller nucleus. Quite condensed chromatin structure without nucleoles
  • Relative plentiful gray blue cytoplasm
  • No granules
 

Orthochromatic Erythroblast

  • 8-12 microns
  • Small round nucleus, often in the center of the cell. Condensed chromatin structure
  • Plentiful reddish cytoplasm with faintly blue components
  • No granules
 

Polychromatic Erythrocyte

  • 7-10 microns
  • No nucleus
  • Cytoplasm reddish with even fainter blue component
  • No granules
 
 

Erythrocyte

  • 6-8 microns
  • No nucleus
  • Faintly reddish cytoplasm,clearing towards the center
  • No granules